HELIOS BioScience
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Features and Benefits

Intended Use

  • Cost-effective, non-xenogeneic / serum substitute
  • Natural source and natural signal for cell growth
  • Abundant growth factors / cytokines / proteins
  • Replace 5 to 20 % FBS with only 2-10% BSSub™-XF
  • Better performance in primary and expansion cultures
  • Lot-to-lot consistency
  • Easy to use and store

BSSub™-XF media supplement supports the growth of a wide variety of both non-adherent and adherent cell lines. BSSub™-XF has twice the potency of fetal bovine serum (i.e. 2-10% of BSSub™-XF media supplement is equivalent to 5-20% fetal bovine serum in the medium).

Turbidity may develop BSSub™-XF cell culture supplement. Experiments have determined that turbidity will not alter the performance of the product.


Cells successfully tested with BSSub™-XF cell culture supplement:

Cancer Cells:
  • Leukemia cell lines (KG-1, K562, JURKAT, HL-60)
  • Hepatocellular carcinoma cell lines (HepG2)
  • Cervical cancer cell lines (HeLa)
  • Breast cancer cell lines (MCF-7)
  • Neuroblastoma cell lines (Neuro-2a)
Other Cells:
  • Corneal epithelial cell
  • Epithelial cell lines (HEK293)
  • Endothelial colony-forming cells (ECFC)
  • Endothelial cell lines (HUVECs, OEC, LEC)
  • Fibroblast
  • Keratinocyte cell lines (HaCaT)
  • Hepatocyte
  • Myoblast cell lines (C2C12)
  • CHO cell
Stem Cells Immune Cells
Sample Request
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