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Frequently Asked Questions
BSSub™-XF

  1. Why use BSSub™-XF as an alternative to fetal bovine serum (FBS)?
  2. Answer: BSSub™-XF is an optimized cell culture supplement containing a natural mixture of human growth factors and cytokines. It can yield superior results versus FBS when culturing many types of cells including adherent and non-adherent cells. Additionally, the potency of BSSub™-XF is about two-fold higher than FBS, and it is therefore a cost-effective choice forcell culture.
  3. What concentration of BSSub™-XF should I use in medium to cultivate cells?
  4. Answer: 5% of BSSub™-XF (v/v) usually exhibits superior results in the cultivation of many types of cells, however, it should be tested to determine the best culture condition for each individual cell type.
  5. How long can frozen BSSub™-XF be stored?
  6. Answer: BSSub™-XF is stable for 2 years from the date of manufacture when stored properly at -20°C. Uninterrupted frozen storage at -20°C for 2 years does not decrease BSSub™-XF performance in cell culture. However, storage at higher temperatures may affect results. We anticipate that our ongoing studies will indicate that the shelf life of BSSub™-XF is longer than 2 years.
  7. What cell types can be grown in BSSub™-XF?
  8. Answer: Our internal testing result and research publications have indicated that BSSub™-XF is able to support fibroblast, CHO cells, corneal epithelial cells, endothelial cell lines, and some cancer cell lines (such as K562, Jurkat, HeLa, and MCF-7).

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