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Frequently Asked Questions
UltraGRO™-Advanced

  1. Why use UltraGRO™-Advanced as an alternative to FBS?
  2. Answer: UltraGRO™-Advanced is a supplement for cell culture that contains a natural mixture of human growth factors including; PDGF, bFGF, EGF, TGF-beta1, VEGF, other cytokines and proteins which yield superior results when culturing many types of human cells, particularly human MSC, regardless of their tissue source. UltraGRO™-Advanced products offer animal protein-free/serum substitutes for replacing FBS and improving your cell culture processes.
  3. What does the cost of UltraGRO™-Advanced compare to FBS?
  4. Answer: FBS is commonly used at 10~20% concentration in cell culture for MSC expansion and UltraGRO™-Advanced products are typically used at 5% concentration, or 1/4th of the volume of FBS used. So when you compare cost, you should therefore compare UltraGRO™-Advanced to 2-4X your current FBS cost. Please see product information.
  5. What is the difference of UltraGRO™-Advanced from UltraGRO™ and other platelet lysate products on the market?
  6. Answer: UltraGRO™-Advanced is manufactured with a patented process to remove coagulation factors (e.g. fibrinogen) which eliminates the need of any additional anti-coagulants when adding UltraGRO™-Advanced to cell culture media.
  7. How and where is UltraGRO™-Advanced processed?
  8. Answer: UltraGRO™-Advanced is manufactured in our Atlanta, GA facility from human platelet units obtained from FDA-registered or HC-registered blood banks in North American. Donors are carefully selected, platelets are fully screened by the blood banks, and then processed by Helios’ proprietary methods and stringent quality standards, and passed through a 0.2 μm filter to produce and fill a sterile bottled product. UltraGRO™-Advanced release criteria include testing for endotoxin, mycoplasma and sterility. A Certificate of Analysis (CoA) is provided with each product shipped from every production lot.
  9. What MSCs have been tested with UltraGRO™-Advanced?
  10. Answer: UltraGRO™-Advanced has been tested internally in the cultivation of human bone marrow derived, adipose tissue derived, and umbilical cord derived MSCs. Our customers and recent research publications have indicated that UltraGRO™-Advanced also works well for culturing human amniotic and cord blood derived MSCs.
  11. Is a GMP-grade UltraGRO™-Advanced available?
  12. Answer: Yes. UltraGRO™-Advanced is available as a GMP product. There are 4 pre-clinical studies and 4 phase I clinical trials are using UltraGRO™-Advanced cultivated human MSCs.
  13. How long can frozen UltraGRO™-Advanced be stored?
  14. Answer: UltraGRO™-Advanced is stable for 2 years from the date of manufacture when stored properly at -20°C. Uninterrupted frozen storage at -20°C for 2 years does not decrease UltraGRO™-Advanced performance in cell culture. However, storage at higher temperatures may affect results. We anticipate that our ongoing studies will indicate that the shelf life of UltraGRO™-Advanced frozen at -20°C is longer than 2 years.

If you want more information, please contact us directly.

 
 
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